Introduction to Fundamental Laboratory Techniques
This module covers essential topics for understanding and mastering basic laboratory skills. The curriculum includes foundational concepts in molecular biology, chemical handling, and laboratory safety, equipping participants with the knowledge and skills necessary for careers in biotechnology and pharmaceutical industries.
Advanced Techniques in Nucleic Acid Handling and AmplificationThis module delves into sophisticated methods for isolating and manipulating nucleic acids, offering a comprehensive understanding of various PCR techniques, gel electrophoresis, DNA/RNA visualization, and sequencing. Participants will gain hands-on experience with advanced protocols essential for genetic analysis and molecular biology research.
Cloning and Expression Systems: Techniques and Applications
This module explores the preparation and use of host cells, the fundamentals of plasmid vectors, and various cloning techniques. Emphasis is placed on understanding the differences between microbial and mammalian vectors, yeast and insect cell expression systems, and advanced cloning methods. Participants will gain practical knowledge in enzyme utilization, primer design, and the latest ligation-independent cloning technologies.
Practicals
Core Laboratory Techniques and Protocols
This training module provides a comprehensive introduction to key laboratory techniques and protocols essential for any research or clinical lab environment. Participants will gain hands-on experience in essential calculations, pipette handling, weighing, and centrifugation, along with proper lab notebook documentation and adherence to quality assurance standards. The course also covers critical procedures such as media preparation and autoclaving, DNA loading dye preparation, buffer preparation and pH adjustment, antibiotic preparation, and bacterial culture techniques. Emphasis is placed on accuracy, reproducibility, and compliance with established laboratory practices.
Nucleic Acid Extraction and Analysis: Advanced Techniques and Protocols
This module explores advanced methods for analysing nucleic acids, focusing on isolation, visualization, and quantification techniques. Participants will also learn procedures for various methods of PCR, RNA manipulation and amplification, preparing them for diverse applications in biological research and diagnostics.
Barcoding and Sequence Analysis Workflow
This module guides participants through the barcoding process, emphasizing comprehensive techniques essential for genetic identification and research applications
Cloning and Expression Techniques
Comprehensive training in fundamental and advanced techniques for molecular cloning and protein expression in bacteria designed to equip participants with the practical skills needed in research and biotechnological applications. Participants will gain practical experience in DNA manipulation, cell transformation, and protein production.
Bioinformatics Training
This training module offers comprehensive instruction in bioinformatics tools for molecular biology applications. Participants will learn primer designing techniques along with the use of bioinformatics tools for DNA analysis and alignment. Additionally, participants will gain proficiency in Sanger sequence data analysis and troubleshooting strategies.
In addition to this, the participants will be trained in developing essential soft skills for laboratory professionals, including experiment planning, timely execution and maintaining data integrity. This training will also enhance critical thinking and troubleshooting abilities crucial for successful scientific endeavours. Additionally, guidance will be provided for effective resume building tailored for careers in the scientific field.