DNA Extraction Kits

Nucleosieve® Plant DNA Extraction Kit

Cat.No.PMD007

Product Description

The Nucleosieve® Plant DNA Extraction Kit is designed for rapid and cost- effective extraction of DNA from the toughest plant tissues including plants with high phenolic content and treated and untreated wood by utilizing silica-based membrane technology in the form of a convenient spin column. This kit utilizes specialized buffers to remove polysaccharides, polyphenolics and other secondary metabolites from plant extracts.

High quality genomic DNA can be extracted from wide variety of fresh, frozen and dried plant species from Leaf, Stem, Root, Dry pepper corns, Processed wood, Normal wood tissue, Flower, Seed, Marine seaweed and also tissues including plants with high polyphenolics as well as treated wood tissues.

Highlights

  • Fast—procedure completed in 40 min following cell lysis
  • Convenient—protocol does not include clogging-prone lysate filtration step
  • High yield—Yields 80% of DNA from plant tissues with A260/A280≥1.8, by purifying 100mg of plant tissues.
  • Compatible—use with awide variety of samples (leaves, roots, sprouts,wood and other parts of various plants).

The standard procedure takes less than 40 minutes following cell lysis and yields purified DNA greater than 30 kb in size. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and the age of the tissue sample. Isolated DNA can be used directly in PCR, qPCR,and other downstream applications.

Applications

  • PCR and qPCR
  • FastDigest and conventional restriction digestion
  • Southern blotting
  • NGS

Note: NucleosieveⓇ Plant DNA Extraction kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Specifications

Product Nucleosieve® Plant DNA Extraction Kit
Catalogue No PMD007
Isolation Technology Spin Column
Sample Type Plants
Product Type Genomic DNA Purification Kit
Test Time Within 40min.
For Use With(Application) Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Sequencing, PCR
Target Genomic DNA
Pack Size 50 Preps,100 Preps,250Preps

Molecular Diagnostics Solutions

Be one of the most recognized source for molecular diagnostic technologies and products

Call Us

+91 484 2915770