Nucleosieve® Stool DNA Extraction Kit
Cat.No.PMD014
GEM Catalogue ID : 5116877-58400757823
Product Description
The Nucleosieve® Stool DNA Extraction Kit is ideal for isolation of DNA from microbial and human cells present in the stool. Stool samples are rich in PCR inhibitors like complex polysaccharides, bile salts, lipids, and urate. In best cases, these inhibitors make targets difficult to amplify. In worst cases, their presence can entirely suppress PCR signals. Therefore, a method to completely remove such inhibitors is critical for any stool sample analysis.This kit utilizes specialized buffers to remove such inhibitors of downstream applications and yields high quality DNA for various applications like microbiome analysis, pathogen detection and genetic research.
Samples are lysed with the aid of ceramic, silica and glass beads. The silica membrane spin column ensures complete removal of all inhibitors of PCR and other enzymatic reactions. The purified DNA is of the highest quality.Nucleosieve® Stool DNA Extraction kit provides the fastest and easiest way to purify stool DNA for reliable use in amplification technologies. This Kit uses 3 different lysis buffers to release DNA, followed by rapid and efficient precipitation of proteins, leaving all nucleic acids in solution.
Highlights
Optimized Components And Protocols
The NucleosieveⓇ Stool DNA Extraction Kit offers improved components and protocols that enhance the traditional spin-column methods. The samples undergoes a specific lysis procedure with an optimized buffer that boosts proteinase K activity and removes protein contamination. A chaotropic salt buffer ensures stable DNA binding to the column resin, while powerful wash buffers effectively eliminate any remaining proteins and salts. Finally, DNA is eluted in a low-salt buffer to stabilize the pH for long-term storage.
Higher Yield And Purity
The improved Nucleosieve Lysis and Wash Buffers ensure consistent generation of purified genomic DNA samples with absorbance ratios around 1.8 for A260/A280, indicating no detectable contamination from protein or guanidine carryover.
Applications
Note:NucleosieveⓇ Stool DNA Extraction kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Specifications
| Product | Nucleosieve® Stool DNA Extraction Kit |
| Catalogue No | PMD014 |
| Isolation Technology | Silica Spin Column |
| Sample Type | Fresh or Frozen Stool Sample |
| For Use With(Application) | Highly Suited For Downstream PCR Applications, Amplification Technologies |
| Pack Size | 50 Preps,100 Preps,250Preps |
| Storage while Shipping | Room Temperature |