RNA Extraction Kits

Nucleosieve® Blood RNA Extraction kits

Cat.No.PMR005

Product Description

Nucleosieve® Blood RNA Extraction kits are designed for rapid and efficient isolation of high quality total cellular RNA from fresh, human whole blood. This kit provide the fastest and easiest way to purify RNA for reliable use in amplification technologies. This Kit uses a lysis buffer, followed by rapid and efficient precipitation of proteins, leaving all nucleic acids in solution.

The kit employs silica-based membrane technology through a simple spin column, completing the entire process under 30 minutes. The resulting purified, high-quality RNA is suitable for various downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-related analyses.

Highlights

  • Efficient – up to 10 μg of total RNA from 200 μL of human blood
  • Convenient – spin columns are capped and assembled with collection tubes
  • Pure – RNA pure from enzyme inhibitors, proteins, ready to use in all demanding applications. Validated in cDNA synthesis and quantitative RT-PCR
  • Fast and simple – procedure takes less than 30 minutes and does not involve organic extraction or differential RBC lysis.
  • Compatible – suitable for vide variety of samples - human and animal blood treated with EDTA, citrate or heparin, buffy coat, bone marrow, and related body fluids.

Applications

  • Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
  • RT-PCR
  • RT-qPCR
  • Northern blotting
  • Nuclease protection assays

Note:NucleosieveⓇ Blood RNA Extraction kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Specifications

Product Nucleosieve® Blood RNA Extraction Kit
Catalogue No PMR005
Isolation Technology Spin Column
Sample Type Blood, Bone Marrow, Buffy Coat
Final Product Type Total RNA
Test Time Within 30min.
For Use With(Application) RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition Room Temperature
Yield 10 μg from 200μl Sample
Pack Size 50 Preps,100 Preps,250Preps

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